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C. glucose 6 phosphate dehydrogenase, the first enzyme in the pentose (5c sugar) phosphate pathway is an irreversible enzyme that converts glucose 6 phosphate into g phosphogluconolatone, D. hexokinase which converts glucose to glucose 6 phosphate and requires 1 ATP but forms 1 NADH and is involved in GLYCOLYSIS and E. Pyruvate kinase vonverts PEP into Pyruvate are all located AWAY from the mitochodniral inside the CYTOPLASM. Answer is C. Citrate allosterically inhibits (-) PFK1, the rate limiting enzyme in glycolysis, which inhibits the conversion of: C. Why Citrate inhibits (decreases) the activity of PFK1.